Reverse-phase HPLC: difference between revisions
Diff·revision 14 → 15·09:05, 9 Jan 2025
Difference between revision 14 and revision 15 of Reverse-phase HPLC. 2 lines changed; the page grew by 362 bytes.
| Revision 14 — 09:04, 26 Dec 2024 Chromatokid (talk) add the reference standard and its traceability 3,884 bytes ±0 | Revision 15 — 09:05, 9 Jan 2025 SecondarySrc_Seb (talk) add category for method validation 4,246 bytes +362 | ||
|---|---|---|---|
| 35 | The technique is also tolerant. Samples can be injected in aqueous solution, the mobile phases are simple and volatile enough to be compatible with [[Mass spectrometry|mass spectrometry]], and the same chemistry scales to [[Preparative HPLC purification|preparative]] loadings. | 35 | The technique is also tolerant. Samples can be injected in aqueous solution, the mobile phases are simple and volatile enough to be compatible with [[Mass spectrometry|mass spectrometry]], and the same chemistry scales to [[Preparative HPLC purification|preparative]] loadings. |
| 36 | 36 | ||
| + | 37 | Its limitations follow from the same properties. Very hydrophobic peptides retain strongly and may need a shorter alkyl chain or a higher organic proportion; acylated analogues such as [[Semaglutide|semaglutide]] fall into this class. Very hydrophilic peptides may not retain at all and elute in the void, where nothing is separated from anything.{{r|usp1503}} | |
| + | 38 | ||
| 37 | == References == | 39 | == References == |
| 38 | {{reflist}} | 40 | {{reflist}} |