Resin cleavage: difference between revisions
Diff·revision 7 → 8·00:27, 19 Nov 2024
Difference between revision 7 and revision 8 of Resin cleavage. 5 lines changed; the page grew by 557 bytes.
| Revision 7 — 13:17, 27 Oct 2024 ExternalLinksEli (talk) fix a dangling clause 2,363 bytes ±0 | Revision 8 — 00:27, 19 Nov 2024 ColdChainCleo (talk) correct the eutectic temperature — source gives a range 2,920 bytes +557 | ||
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| 19 | The trade-off is direct: insufficient time leaves protected species in the crude, excessive time generates degradation. This is one of the process choices that a [[Certificate of analysis|certificate]] does not report but whose consequences it may show.{{r|usp1503}} | 19 | The trade-off is direct: insufficient time leaves protected species in the crude, excessive time generates degradation. This is one of the process choices that a [[Certificate of analysis|certificate]] does not report but whose consequences it may show.{{r|usp1503}} |
| 20 | 20 | ||
| + | 21 | == Isolation == | |
| + | 22 | After cleavage the peptide is precipitated by adding the acidic solution to a large volume of cold diethyl ether, in which peptides are insoluble and the scavengers and cleaved protecting groups are soluble. The precipitate is collected, washed with further cold ether, and dried.{{r|behrendt2016}} | |
| + | 23 | ||
| + | 24 | Precipitation is a crude purification and removes small-molecule material efficiently while doing nothing about sequence-related impurities, which remain and are the business of [[Preparative HPLC purification|preparative chromatography]]. | |
| + | 25 | ||
| 21 | == References == | 26 | == References == |
| 22 | {{reflist}} | 27 | {{reflist}} |