Reconstitution of lyophilised peptides (revision 1)
Old revision·06:11, 21 Jun 2024·COA_Colwyn
This is an old revision of this page, as it stood at 06:11, 21 Jun 2024, saved by COA_Colwyn with the summary new article: dosing and handling, expansion welcome. It may differ substantially from the current revision, and any error it contains may since have been corrected.
| Reconstitution of lyophilised peptides | |
|---|---|
| Reverses | Lyophilisation |
| Common diluents | Bacteriostatic water, Sterile water for injection |
| Consequence | Solution stability is far shorter than powder stability |
| Analytical method infobox · conventions | |
Reconstitution is the dissolution of a lyophilised peptide cake in a liquid diluent. The dried solid is the stable form: once in solution the peptide is exposed to hydrolysis, deamidation, oxidation and aggregation, all of which proceed far faster in the liquid phase.[1]
Two quantities determine the resulting concentration: the mass of peptide actually present in the vial, and the volume of diluent added. The first is not the number printed on the label unless content, water and fill mass are known — a vial nominally containing 5 mg may contain appreciably less peptide. See Underfilling.[2]
References
Categories: