Reconstitution of lyophilised peptides: difference between revisions
Diff·revision 1 → 2·20:27, 23 Jun 2024
Difference between revision 1 and revision 2 of Reconstitution of lyophilised peptides. 5 lines changed; the page grew by 584 bytes.
| Revision 1 — 06:11, 21 Jun 2024 COA_Colwyn (talk) new article: dosing and handling, expansion welcome 1,487 bytes +1,487 | Revision 2 — 20:27, 23 Jun 2024 DrTitration (talk) state the syringe graduation the unit figure assumes 2,071 bytes +584 | ||
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| 10 | Two quantities determine the resulting concentration: the mass of peptide actually present in the vial, and the volume of diluent added. The first is not the number printed on the label unless [[Peptide content|content]], water and fill mass are known — a vial nominally containing 5 mg may contain appreciably less peptide. See [[Underfilling]].{{r|usp1503}} | 10 | Two quantities determine the resulting concentration: the mass of peptide actually present in the vial, and the volume of diluent added. The first is not the number printed on the label unless [[Peptide content|content]], water and fill mass are known — a vial nominally containing 5 mg may contain appreciably less peptide. See [[Underfilling]].{{r|usp1503}} |
| 11 | 11 | ||
| + | 12 | == Mechanics == | |
| + | 13 | Diluent is directed against the vial wall rather than onto the cake. A jet striking the cake dissolves it violently, generating the air–liquid interfaces and shear at which peptides unfold and aggregate; running the liquid down the wall lets the cake dissolve from beneath.{{r|manning2010}} | |
| + | 14 | ||
| + | 15 | Dissolution is completed by gentle swirling or by leaving the vial to stand. Shaking is the practice most likely to produce visible haze, for the same interfacial reason. A cake that has not fully dissolved after several minutes should be given time rather than agitation. | |
| + | 16 | ||
| 12 | == References == | 17 | == References == |
| 13 | {{reflist}} | 18 | {{reflist}} |