Preparative HPLC purification: difference between revisions
Diff·revision 16 → 17·00:53, 27 Apr 2025
Difference between revision 16 and revision 17 of Preparative HPLC purification. 5 lines changed; the page grew by 696 bytes.
| Revision 16 — 00:28, 5 Apr 2025 DiffWatchDov (talk) British spelling per PP:MOS 3,866 bytes ±0 | Revision 17 — 00:53, 27 Apr 2025 CoI_Corin (talk) give the closure integrity test by name 4,562 bytes +696 | ||
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| 28 | Residual acetonitrile from the mobile phase is the principal [[Residual solvent|residual solvent]] of concern in peptides purified this way, and its removal is a function of the drying step rather than of the chromatography.{{r|usp467}} | 28 | Residual acetonitrile from the mobile phase is the principal [[Residual solvent|residual solvent]] of concern in peptides purified this way, and its removal is a function of the drying step rather than of the chromatography.{{r|usp467}} |
| 29 | 29 | ||
| + | 30 | == Why analytical results can flatter preparative material == | |
| + | 31 | An analytical method derived from the preparative separation shares its selectivity. Species that co-eluted with the product during purification will co-elute with it during analysis, and will not appear as impurities.{{r|usp621}} | |
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| + | 33 | This is not a criticism of any particular practice — using the same chemistry is the obvious first choice — but it means that a purity figure from a method related to the purification is a weaker claim than one from an orthogonal method. Orthogonality can come from a different stationary-phase chemistry, a different modifier, a different pH, or a different mode altogether such as ion-exchange. | |
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| 30 | == References == | 35 | == References == |
| 31 | {{reflist}} | 36 | {{reflist}} |