Liquid chromatography-mass spectrometry: difference between revisions
Diff·revision 1 → 2·06:31, 21 Sep 2024
Difference between revision 1 and revision 2 of Liquid chromatography-mass spectrometry. 6 lines changed; the page grew by 785 bytes.
| Revision 1 — 17:54, 18 Sep 2024 MassSpecMarv (talk) new article: analytical method, expansion welcome 1,347 bytes +1,347 | Revision 2 — 06:31, 21 Sep 2024 MetricMabel (talk) give the limit of quantitation with the signal-to-noise ratio it assumes 2,132 bytes +785 | ||
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| 11 | The interface is almost always [[Electrospray ionisation|electrospray]], which works directly from flowing solution. This compatibility is what made the technique routine, and it constrains the chromatography: mobile phases must be volatile and free of non-volatile buffers.{{r|fenn1989}} | 11 | The interface is almost always [[Electrospray ionisation|electrospray]], which works directly from flowing solution. This compatibility is what made the technique routine, and it constrains the chromatography: mobile phases must be volatile and free of non-volatile buffers.{{r|fenn1989}} |
| 12 | 12 | ||
| + | 13 | == What it adds over either alone == | |
| + | 14 | Chromatography alone establishes that one species dominates; mass spectrometry alone establishes that a species of the expected mass is present. Together they establish that the dominant species has the expected mass, which is a materially stronger statement than either.{{r|aebersold2003}} | |
| + | 15 | ||
| + | 16 | Impurity identification is where the combination is most valuable. A deletion sequence appears as a peak of lower mass by one residue; an oxidised species by 16 Da more; an incompletely deprotected species by the mass of the retained group. See [[Resin cleavage]] and [[Solid-phase peptide synthesis]].{{r|usp1503}} | |
| + | 17 | ||
| 13 | == References == | 18 | == References == |
| 14 | {{reflist}} | 19 | {{reflist}} |
| 15 | <ref name="aebersold2003">Aebersold R, Mann M. "Mass spectrometry-based proteomics." ''Nature'' 422(6928):198–207 (2003). PMID 12634793.</ref> | 20 | <ref name="aebersold2003">Aebersold R, Mann M. "Mass spectrometry-based proteomics." ''Nature'' 422(6928):198–207 (2003). PMID 12634793.</ref> |
| 16 | <ref name="fenn1989">Fenn JB, Mann M, Meng CK, Wong SF, Whitehouse CM. "Electrospray ionization for mass spectrometry of large biomolecules." ''Science'' 246(4926):64–71 (1989). PMID 2675315.</ref> | 21 | <ref name="fenn1989">Fenn JB, Mann M, Meng CK, Wong SF, Whitehouse CM. "Electrospray ionization for mass spectrometry of large biomolecules." ''Science'' 246(4926):64–71 (1989). PMID 2675315.</ref> |
| + | 22 | <ref name="usp1503">United States Pharmacopeia, General Chapter <1503>, ''Quality Attributes of Synthetic Peptide Drug Substances''.</ref> | |
| 17 | 23 | ||
| 18 | {{DEFAULTSORT:Liquid chromatography-mass spectrometry}} | 24 | {{DEFAULTSORT:Liquid chromatography-mass spectrometry}} |