Electrospray ionisation: difference between revisions
Diff·revision 14 → 15·08:24, 31 May 2025
Difference between revision 14 and revision 15 of Electrospray ionisation. 5 lines changed; the page grew by 561 bytes.
| Revision 14 — 13:19, 1 May 2025 IsocraticIla (talk) add the ionisation mode to the mass-spectrometry description 3,512 bytes ±0 | Revision 15 — 08:24, 31 May 2025 MergeMorwenna (talk) add the forced-degradation conditions the claim rests on 4,073 bytes +561 | ||
|---|---|---|---|
| 29 | The multiply charged envelope carries information beyond mass: its distribution shifts with conformation, so a partially unfolded protein produces a different envelope from a folded one. For small peptides this is of little consequence.{{r|fenn1989}} | 29 | The multiply charged envelope carries information beyond mass: its distribution shifts with conformation, so a partially unfolded protein produces a different envelope from a folded one. For small peptides this is of little consequence.{{r|fenn1989}} |
| 30 | 30 | ||
| + | 31 | == What a reported mass means == | |
| + | 32 | A deconvolved mass on a certificate is the software's estimate of the neutral monoisotopic or average mass from the observed charge envelope. Whether the instrument could resolve small differences depends on the analyser — see [[Mass spectrometry]] — and a report that does not name the instrument does not establish what could have been seen.{{r|aebersold2003}} | |
| + | 33 | ||
| + | 34 | Poorly ionising species may be absent from a spectrum while present in the material, so absence from the spectrum is not absence from the sample.{{r|usp1503}} | |
| + | 35 | ||
| 31 | == References == | 36 | == References == |
| 32 | {{reflist}} | 37 | {{reflist}} |