Bacterial endotoxin test: difference between revisions
Diff·revision 1 → 2·13:20, 1 Sep 2024
Difference between revision 1 and revision 2 of Bacterial endotoxin test. 5 lines changed; the page grew by 691 bytes.
| Revision 1 — 22:34, 27 Aug 2024 SarcopeniaSefa (talk) create article — method stub 1,436 bytes +1,436 | Revision 2 — 13:20, 1 Sep 2024 LedeLeander (talk) clarify that area percent is not mass percent 2,127 bytes +691 | ||
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| 11 | Endotoxin is not a living organism and is not removed by sterilisation. A preparation can be sterile and pyrogenic at the same time: autoclaving kills bacteria but leaves their lipopolysaccharide intact and biologically active. Endotoxin is the commonest but not the only pyrogen, and a separate rabbit pyrogen test exists for the wider class.{{r|usp151}} This is why endotoxin and [[Sterility testing|sterility]] are separate determinations answering separate questions.{{r|usp85}} | 11 | Endotoxin is not a living organism and is not removed by sterilisation. A preparation can be sterile and pyrogenic at the same time: autoclaving kills bacteria but leaves their lipopolysaccharide intact and biologically active. Endotoxin is the commonest but not the only pyrogen, and a separate rabbit pyrogen test exists for the wider class.{{r|usp151}} This is why endotoxin and [[Sterility testing|sterility]] are separate determinations answering separate questions.{{r|usp85}} |
| 12 | 12 | ||
| + | 13 | == Methods == | |
| + | 14 | Three variants are recognised. The gel-clot method observes whether a firm clot forms at a given dilution and gives a limit result rather than a number. The turbidimetric method follows the increase in turbidity as the cascade proceeds and is quantitative. The chromogenic method measures release of a coloured product from a synthetic substrate and is also quantitative.{{r|usp85}} | |
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| + | 16 | Recombinant factor C assays, which use a recombinant version of the initiating enzyme rather than crab-derived lysate, have been introduced and are increasingly accepted. They avoid the animal-derived reagent and are more specific, since they respond only to endotoxin and not to β-glucans. | |
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| 13 | == References == | 18 | == References == |
| 14 | {{reflist}} | 19 | {{reflist}} |