Area percent purity: difference between revisions
Diff·revision 11 → 12·16:38, 4 Jan 2025
Difference between revision 11 and revision 12 of Area percent purity. 5 lines changed; the page grew by 245 bytes.
| Revision 11 — 03:24, 16 Dec 2024 IsocraticIla (talk) split §Principle from §Practical limitations 5,069 bytes +1,470 | Revision 12 — 16:38, 4 Jan 2025 FreightFenna (talk) add the reference standard and its traceability 5,314 bytes +245 | ||
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| 1 | {{Infobox method | 1 | {{Infobox method |
| 2 | | name = Area percent purity | 2 | | name = Area percent purity |
| + | 3 | | subtitle = Chromatographic measure | |
| 3 | | image = chromatogram.svg | 4 | | image = chromatogram.svg |
| 4 | | caption = Principal peak and resolved impurities. Area percent is the shaded peak as a proportion of everything integrated — not as a proportion of what was weighed into the vial. | 5 | | caption = Principal peak and resolved impurities. Area percent is the shaded peak as a proportion of everything integrated — not as a proportion of what was weighed into the vial. |
| ⋮ | ⋮ | ||
| 7 | | Units = Per cent of total integrated area | 8 | | Units = Per cent of total integrated area |
| 8 | | Typical method = Reverse-phase HPLC with ultraviolet detection | 9 | | Typical method = Reverse-phase HPLC with ultraviolet detection |
| + | 10 | <!-- Depends on --> | |
| + | 11 | | Detection = Response is not uniform across species | |
| + | 12 | | Integration = Valley-to-valley and drop-line give different answers | |
| + | 13 | | Gradient = Determines what co-elutes and what resolves | |
| 9 | }} | 14 | }} |
| 10 | {{hatnote|For the mass fraction of a vial that is peptide, which is a different quantity, see [[Peptide content]].}} | 15 | {{hatnote|For the mass fraction of a vial that is peptide, which is a different quantity, see [[Peptide content]].}} |