Amino acid analysis: difference between revisions
Diff·revision 4 → 5·16:13, 24 Dec 2024
Difference between revision 4 and revision 5 of Amino acid analysis. 3 lines changed; the page grew by 254 bytes.
| Revision 4 — 17:42, 12 Dec 2024 AAA_Analyst (talk) add the cross-link to the compendial chapter 2,367 bytes ±0 | Revision 5 — 16:13, 24 Dec 2024 AnalyticalAnnie (talk) copyedit 2,621 bytes +254 | ||
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| 11 | It is one of the few methods that gives content without requiring a [[Reference standard|reference standard]] of the peptide itself, because the standards required are the individual amino acids.{{r|usp11}} This makes it particularly valuable for compounds with no marketed product and therefore no compendial standard.{{r|usp1503}} | 11 | It is one of the few methods that gives content without requiring a [[Reference standard|reference standard]] of the peptide itself, because the standards required are the individual amino acids.{{r|usp11}} This makes it particularly valuable for compounds with no marketed product and therefore no compendial standard.{{r|usp1503}} |
| 12 | 12 | ||
| + | 13 | The method is destructive, laborious and subject to well-characterised systematic errors, which is why it is used to qualify a standard or to establish content on a lot rather than as a routine release test.{{r|usp1052}} | |
| + | 14 | ||
| 13 | == Procedure == | 15 | == Procedure == |
| 14 | A weighed sample is hydrolysed, conventionally in 6 M hydrochloric acid at 110 °C for 24 hours under vacuum or inert atmosphere. The hydrolysate is derivatised and the amino acids separated and quantified by chromatography against standards of each.{{r|usp1052}} | 16 | A weighed sample is hydrolysed, conventionally in 6 M hydrochloric acid at 110 °C for 24 hours under vacuum or inert atmosphere. The hydrolysate is derivatised and the amino acids separated and quantified by chromatography against standards of each.{{r|usp1052}} |
| ⋮ | ⋮ | ||
| 27 | [[Category:Analytical methods]] | 29 | [[Category:Analytical methods]] |
| 28 | [[Category:Reference standards]] | 30 | [[Category:Reference standards]] |
| + | 31 | [[Category:Analytical science]] | |
| 29 | 32 |