Amino acid analysis: difference between revisions
Diff·revision 2 → 3·08:19, 29 Nov 2024
Difference between revision 2 and revision 3 of Amino acid analysis. 2 lines changed; the page grew by 324 bytes.
| Revision 2 — 03:34, 25 Nov 2024 COA_Colwyn (talk) correct the integration description — valley-to-valley, not drop-line 2,043 bytes ±0 | Revision 3 — 08:19, 29 Nov 2024 TirzTaxonomist (talk) split §Principle from §Practical limitations 2,367 bytes +324 | ||
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| 16 | The molar ratios obtained are compared with those expected from the sequence. Agreement supports identity, though it does not establish sequence order — a permutation of the same residues gives the same composition, which is why composition is complementary to [[Mass spectrometry|mass]] and fragmentation rather than a substitute for them. | 16 | The molar ratios obtained are compared with those expected from the sequence. Agreement supports identity, though it does not establish sequence order — a permutation of the same residues gives the same composition, which is why composition is complementary to [[Mass spectrometry|mass]] and fragmentation rather than a substitute for them. |
| 17 | 17 | ||
| + | 18 | Absolute content follows from the total recovered amino acid mass against the sample mass taken. This is the figure that makes the method valuable for quantitation, and it is unaffected by water, counterion or residual solvent, all of which contribute to the weighed mass and none of which yields amino acids.{{r|usp1503}} | |
| + | 19 | ||
| 18 | == References == | 20 | == References == |
| 19 | {{reflist}} | 21 | {{reflist}} |