Amino acid analysis: difference between revisions
Diff·revision 10 → 11·03:23, 18 Apr 2025
Difference between revision 10 and revision 11 of Amino acid analysis. 2 lines changed; the page grew by 335 bytes.
| Revision 10 — 21:55, 27 Mar 2025 TFA_Counterion (talk) state the mass accuracy in parts per million rather than daltons 3,703 bytes +121 | Revision 11 — 03:23, 18 Apr 2025 DiluentDiya (talk) convert the method-comparison paragraph to a table 4,038 bytes +335 | ||
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| 32 | These are not defects of a particular laboratory; they are properties of acid hydrolysis and are handled by convention. Serine and threonine losses are corrected by hydrolysing for several durations and extrapolating to zero time; valine and isoleucine by extending hydrolysis. Both corrections are established procedures rather than ad hoc adjustments.{{r|usp1052,ich_q2}} | 32 | These are not defects of a particular laboratory; they are properties of acid hydrolysis and are handled by convention. Serine and threonine losses are corrected by hydrolysing for several durations and extrapolating to zero time; valine and isoleucine by extending hydrolysis. Both corrections are established procedures rather than ad hoc adjustments.{{r|usp1052,ich_q2}} |
| 33 | 33 | ||
| + | 34 | The practical consequence is that an amino acid analysis result should be read with the hydrolysis conditions in view, and that residues known to be destroyed are simply not evidence either way. A composition report that lists tryptophan at its theoretical value from a standard hydrolysis is reporting something that did not happen. | |
| + | 35 | ||
| 34 | == References == | 36 | == References == |
| 35 | {{reflist}} | 37 | {{reflist}} |