Solid-phase peptide synthesis: difference between revisions
Diff·revision 5 → 6·19:37, 31 Jul 2024
Difference between revision 5 and revision 6 of Solid-phase peptide synthesis. 2 lines changed; the page grew by 361 bytes.
| Revision 5 — 16:40, 17 Jul 2024 PrepHPLC_Pia (talk) add the crimp-seal step to the fill-and-finish description 1,893 bytes ±0 | Revision 6 — 19:37, 31 Jul 2024 SemaglutideSasha (talk) rm the vendor-specific packaging detail; not general enough for the article 2,254 bytes +361 | ||
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| 16 | The linker determines the C-terminal functionality. Wang and related linkers give a free acid on cleavage; Rink amide linkers give a C-terminal amide. Since several therapeutic peptides are C-terminally amidated, linker choice is a specification-level decision rather than a convenience. | 16 | The linker determines the C-terminal functionality. Wang and related linkers give a free acid on cleavage; Rink amide linkers give a C-terminal amide. Since several therapeutic peptides are C-terminally amidated, linker choice is a specification-level decision rather than a convenience. |
| 17 | 17 | ||
| + | 18 | Resin loading — millimoles of site per gram — sets the scale and also affects chain crowding. High loading gives more material per batch but increases the chance of interchain aggregation on the support, which is the main cause of difficult couplings. See [[Peptide aggregation]] for the solution-phase analogue of the same phenomenon.{{r|merrifield1963}} | |
| + | 19 | ||
| 18 | == References == | 20 | == References == |
| 19 | {{reflist}} | 21 | {{reflist}} |