Solid-phase peptide synthesis: difference between revisions
Diff·revision 24 → 25·02:02, 1 Jun 2025
Difference between revision 24 and revision 25 of Solid-phase peptide synthesis. 2 lines changed; the page grew by 252 bytes.
| Revision 24 — 20:51, 9 May 2025 GHK_Gwendolen (talk) state that a phase-change pack and a gel pack behave differently 5,107 bytes ±0 | Revision 25 — 02:02, 1 Jun 2025 RegainRomilly (talk) convert the storage conditions to a table 5,359 bytes +252 | ||
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| 43 | For long sequences a fully stepwise synthesis becomes impractical and fragment condensation is used: shorter segments are made on solid phase, cleaved, purified by chromatography, and joined in solution.{{r|usp621}} This adds purification steps but recovers yield by removing deletion sequences before they can accumulate. See [[Liquid-phase peptide synthesis]]. | 43 | For long sequences a fully stepwise synthesis becomes impractical and fragment condensation is used: shorter segments are made on solid phase, cleaved, purified by chromatography, and joined in solution.{{r|usp621}} This adds purification steps but recovers yield by removing deletion sequences before they can accumulate. See [[Liquid-phase peptide synthesis]]. |
| 44 | 44 | ||
| + | 45 | Where a supplier's process sits on this spectrum is not usually disclosed and is not derivable from a certificate. What is derivable, from an itemised related-substances table, is something about how well the process worked for that lot.{{r|usp1503}} | |
| + | 46 | ||
| 45 | == References == | 47 | == References == |
| 46 | {{reflist}} | 48 | {{reflist}} |