Solid-phase peptide synthesis: difference between revisions
Diff·revision 16 → 17·20:54, 30 Dec 2024
Difference between revision 16 and revision 17 of Solid-phase peptide synthesis. 2 lines changed; the page grew by 280 bytes.
| Revision 16 — 10:54, 12 Dec 2024 CustomsClarke (talk) add the cross-link to the temperature-excursion article 3,999 bytes +36 | Revision 17 — 20:54, 30 Dec 2024 CounterfeitCato (talk) clarify that "cold chain" without a logger is a claim, not a record 4,279 bytes +280 | ||
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| 36 | These are process choices rather than quality attributes, and they do not appear on a [[Certificate of analysis|certificate]]. Their consequences do: a sequence handled poorly gives a crude with a characteristic deletion at the difficult position, which a sufficiently specific method will resolve as a distinct related substance.{{r|usp1503}} | 36 | These are process choices rather than quality attributes, and they do not appear on a [[Certificate of analysis|certificate]]. Their consequences do: a sequence handled poorly gives a crude with a characteristic deletion at the difficult position, which a sufficiently specific method will resolve as a distinct related substance.{{r|usp1503}} |
| 37 | 37 | ||
| + | 38 | Racemisation is a separate risk, concentrated at histidine and cysteine and at any residue activated for too long before coupling. It is invisible to [[Mass spectrometry|mass spectrometry]], since the epimer is isobaric, and requires a chiral method to detect.{{r|behrendt2016}} | |
| + | 39 | ||
| 38 | == References == | 40 | == References == |
| 39 | {{reflist}} | 41 | {{reflist}} |