Retention time: difference between revisions
Diff·revision 9 → 10·18:51, 6 Jan 2025
Difference between revision 9 and revision 10 of Retention time. 8 lines changed; the page grew by 374 bytes.
| Revision 9 — 22:28, 21 Dec 2024 CiteBot (talk) bot: repair redlinked category 2,932 bytes +31 | Revision 10 — 18:51, 6 Jan 2025 SatietySunniva (talk) expand §What it can identify 3,306 bytes +374 | ||
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| 32 | Dwell-volume differences are the reason a method transferred between instruments often needs adjustment: the delay between the pump forming a gradient and it reaching the column differs, so retention shifts even with identical settings.{{r|usp621}} | 32 | Dwell-volume differences are the reason a method transferred between instruments often needs adjustment: the delay between the pump forming a gradient and it reaching the column differs, so retention shifts even with identical settings.{{r|usp621}} |
| 33 | 33 | ||
| + | 34 | Because of all this, a retention time quoted on a certificate is a within-system value. It is worth reporting — it lets a repeat determination be checked for gross discrepancy — but it is not a transferable identifier.{{r|usp1503}} | |
| + | 35 | ||
| 34 | == References == | 36 | == References == |
| 35 | {{reflist}} | 37 | {{reflist}} |
| ⋮ | ⋮ | ||
| 37 | <ref name="usp1503">United States Pharmacopeia, General Chapter <1503>, ''Quality Attributes of Synthetic Peptide Drug Substances''.</ref> | 39 | <ref name="usp1503">United States Pharmacopeia, General Chapter <1503>, ''Quality Attributes of Synthetic Peptide Drug Substances''.</ref> |
| 38 | <ref name="mant1996">Mant CT, Hodges RS. "Analysis of peptides by high-performance liquid chromatography." ''Methods in Enzymology'' 271:3–50 (1996). PMID 8782429.</ref> | 40 | <ref name="mant1996">Mant CT, Hodges RS. "Analysis of peptides by high-performance liquid chromatography." ''Methods in Enzymology'' 271:3–50 (1996). PMID 8782429.</ref> |
| + | 41 | ||
| + | 42 | == See also == | |
| + | 43 | * [[Reverse-phase HPLC]] | |
| + | 44 | * [[High-performance liquid chromatography]] | |
| + | 45 | * [[Reference standard]] | |
| + | 46 | * [[Area percent purity]] | |
| 39 | 47 | ||
| 40 | {{DEFAULTSORT:Retention time}} | 48 | {{DEFAULTSORT:Retention time}} |