Preparative HPLC purification (revision 3)
Old revision·22:34, 15 Aug 2024·CrudePeptidePearl
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| Preparative HPLC purification | |
|---|---|
| Objective | Collect a fraction, not measure one |
| Scale | Grams to kilograms per run |
| Trade-off | Yield against purity |
| Analytical method infobox · conventions | |
Preparative HPLC purification applies the chromatography of reverse-phase separation at a scale intended to collect material rather than to analyse it. For synthetic peptides it is the step that converts a crude product into a usable one, and it is typically the most expensive stage of manufacture.[1]
The governing trade-off is yield against purity. Collecting a narrow window around the peak apex gives high purity and discards a substantial fraction of the product; collecting more widely recovers more material and includes more of the shoulders where impurities elute. Where the cut is made is a commercial decision as much as a technical one.[2]
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