Preparative HPLC purification: difference between revisions
Diff·revision 11 → 12·13:45, 8 Jan 2025
Difference between revision 11 and revision 12 of Preparative HPLC purification. 9 lines changed; the page grew by 453 bytes.
| Revision 11 — 06:28, 20 Dec 2024 CounterfeitCato (talk) expand §Scale-up 3,390 bytes ±0 | Revision 12 — 13:45, 8 Jan 2025 RepackRadek (talk) rm the shipping recommendation — that is advice, not description 3,843 bytes +453 | ||
|---|---|---|---|
| 26 | Solvent is removed and the peptide isolated, usually by [[Lyophilisation|lyophilisation]]. Because the mobile phase contains trifluoroacetic acid, the isolated solid is a [[Trifluoroacetate counterion|trifluoroacetate salt]] unless a salt-exchange step is performed, and the counterion contributes mass that is not peptide — see [[Peptide content]]. | 26 | Solvent is removed and the peptide isolated, usually by [[Lyophilisation|lyophilisation]]. Because the mobile phase contains trifluoroacetic acid, the isolated solid is a [[Trifluoroacetate counterion|trifluoroacetate salt]] unless a salt-exchange step is performed, and the counterion contributes mass that is not peptide — see [[Peptide content]]. |
| 27 | 27 | ||
| + | 28 | Residual acetonitrile from the mobile phase is the principal [[Residual solvent|residual solvent]] of concern in peptides purified this way, and its removal is a function of the drying step rather than of the chromatography.{{r|usp467}} | |
| + | 29 | ||
| 28 | == References == | 30 | == References == |
| 29 | {{reflist}} | 31 | {{reflist}} |
| ⋮ | ⋮ | ||
| 31 | <ref name="mant1996">Mant CT, Hodges RS. "Analysis of peptides by high-performance liquid chromatography." ''Methods in Enzymology'' 271:3–50 (1996). PMID 8782429.</ref> | 33 | <ref name="mant1996">Mant CT, Hodges RS. "Analysis of peptides by high-performance liquid chromatography." ''Methods in Enzymology'' 271:3–50 (1996). PMID 8782429.</ref> |
| 32 | <ref name="usp621">United States Pharmacopeia, General Chapter <621>, ''Chromatography''.</ref> | 34 | <ref name="usp621">United States Pharmacopeia, General Chapter <621>, ''Chromatography''.</ref> |
| + | 35 | <ref name="usp467">United States Pharmacopeia, General Chapter <467>, ''Residual Solvents''.</ref> | |
| + | 36 | ||
| + | 37 | == See also == | |
| + | 38 | * [[Reverse-phase HPLC]] | |
| + | 39 | * [[Crude peptide]] | |
| + | 40 | * [[Lyophilisation]] | |
| + | 41 | * [[Trifluoroacetate counterion]] | |
| 33 | 42 | ||
| 34 | {{DEFAULTSORT:Preparative HPLC purification}} | 43 | {{DEFAULTSORT:Preparative HPLC purification}} |