Peptide synthesis: difference between revisions
Diff·revision 5 → 6·00:16, 6 Aug 2024
Difference between revision 5 and revision 6 of Peptide synthesis. 2 lines changed; the page grew by 411 bytes.
| Revision 5 — 23:39, 24 Jul 2024 Chromatokid (talk) add the shelf temperature and the primary drying step to the cycle description 2,347 bytes +311 | Revision 6 — 00:16, 6 Aug 2024 IncretinIvo (talk) state the difference between a validated shipper and an insulated box 2,758 bytes +411 | ||
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| 10 | Chemical synthesis is the only practical route for peptides containing non-proteinogenic residues, and almost every engineered therapeutic peptide contains at least one — the α-aminoisobutyric acid of [[Semaglutide|semaglutide]] and [[Tirzepatide|tirzepatide]] cannot be introduced by a ribosome.{{r|behrendt2016}} | 10 | Chemical synthesis is the only practical route for peptides containing non-proteinogenic residues, and almost every engineered therapeutic peptide contains at least one — the α-aminoisobutyric acid of [[Semaglutide|semaglutide]] and [[Tirzepatide|tirzepatide]] cannot be introduced by a ribosome.{{r|behrendt2016}} |
| 11 | 11 | ||
| + | 12 | Three approaches exist: [[Solid-phase peptide synthesis|solid-phase synthesis]], in which the growing chain is anchored to an insoluble support; [[Liquid-phase peptide synthesis|solution-phase synthesis]], in which it is not; and hybrid approaches in which fragments made on solid phase are joined in solution. Solid phase dominates for research quantities and for most therapeutic peptides.{{r|behrendt2016}} | |
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| 12 | == The elementary cycle == | 14 | == The elementary cycle == |
| 13 | Each residue is added by a two-step cycle. The N-terminal protecting group of the growing chain is removed, then the next amino acid — protected at its own N-terminus and on any reactive side chain — is activated and coupled.{{r|merrifield1963}} | 15 | Each residue is added by a two-step cycle. The N-terminal protecting group of the growing chain is removed, then the next amino acid — protected at its own N-terminus and on any reactive side chain — is activated and coupled.{{r|merrifield1963}} |