Mass spectrometry: difference between revisions
Diff·revision 23 → 24·21:32, 4 Jun 2025
Difference between revision 23 and revision 24 of Mass spectrometry. 2 lines changed; the page grew by 364 bytes.
| Revision 23 — 13:46, 12 May 2025 IsocraticIla (talk) add the forced-degradation conditions the claim rests on 5,248 bytes ±0 | Revision 24 — 21:32, 4 Jun 2025 ChromCleanupCass (talk) rm the vendor name from a methodological sentence 5,612 bytes +364 | ||
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| 39 | Where quantification is required, the usual approach couples chromatography to mass spectrometry — [[Liquid chromatography-mass spectrometry|LC-MS]] — and calibrates against a [[Reference standard|reference standard]], ideally with an isotopically labelled internal standard. This is standard practice in bioanalysis and is not typical of the certificates seen in research-chemical supply; a quantitative claim requires a validated procedure behind it.{{r|ich_q2}} | 39 | Where quantification is required, the usual approach couples chromatography to mass spectrometry — [[Liquid chromatography-mass spectrometry|LC-MS]] — and calibrates against a [[Reference standard|reference standard]], ideally with an isotopically labelled internal standard. This is standard practice in bioanalysis and is not typical of the certificates seen in research-chemical supply; a quantitative claim requires a validated procedure behind it.{{r|ich_q2}} |
| 40 | 40 | ||
| + | 41 | The practical consequence is that a mass spectrum on a certificate answers "is this the right molecule" and nothing else. It does not report purity, does not report [[Peptide content|content]], and does not establish that minor species absent from the spectrum are absent from the material — poorly ionising impurities may simply not appear.{{r|aebersold2003}} | |
| + | 42 | ||
| 41 | == References == | 43 | == References == |
| 42 | {{reflist}} | 44 | {{reflist}} |