High-performance liquid chromatography: difference between revisions
Diff·revision 6 → 7·01:24, 16 Aug 2024
Difference between revision 6 and revision 7 of High-performance liquid chromatography. 6 lines changed; the page grew by 270 bytes.
| Revision 6 — 06:20, 4 Aug 2024 GradientGus (talk) state that ultraviolet response is not uniform across impurities 2,909 bytes +289 | Revision 7 — 01:24, 16 Aug 2024 ForestPlotFinn (talk) expand §What the chromatogram reports 3,179 bytes +270 | ||
|---|---|---|---|
| 4 | | Separates by = Differential partition between stationary and mobile phases | 4 | | Separates by = Differential partition between stationary and mobile phases |
| 5 | | Usual detector for peptides = UV absorbance at 214 nm | 5 | | Usual detector for peptides = UV absorbance at 214 nm |
| + | 6 | <!-- Key parameters --> | |
| + | 7 | | Column = Chemistry, particle size, length, internal diameter | |
| + | 8 | | Mobile phase = Aqueous and organic components, modifier | |
| + | 9 | | Gradient = Composition change over time | |
| + | 10 | | Flow rate = Typically 0.2–1.5 mL/min | |
| 6 | }} | 11 | }} |
| 7 | 12 | ||
| ⋮ | ⋮ | ||
| 28 | [[Category:Chromatography]] | 33 | [[Category:Chromatography]] |
| 29 | [[Category:Analytical methods]] | 34 | [[Category:Analytical methods]] |
| + | 35 | [[Category:Analytical science]] | |
| 30 | 36 |