Electrospray ionisation: difference between revisions
Diff·revision 4 → 5·16:16, 20 Oct 2024
Difference between revision 4 and revision 5 of Electrospray ionisation. 3 lines changed; the page grew by 206 bytes.
| Revision 4 — 11:52, 2 Oct 2024 TechnicianTeal (talk) correct the integration description — valley-to-valley, not drop-line 1,979 bytes ±0 | Revision 5 — 16:16, 20 Oct 2024 KarlFischerKit (talk) add the detection wavelength 2,185 bytes +206 | ||
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| 11 | It is a soft technique — the molecule survives largely intact — and it produces multiply charged ions from peptides and proteins, so a large molecule appears at a mass-to-charge ratio within the range of ordinary analysers. A single peptide therefore produces a series of peaks at successive charge states, deconvolved by software into one neutral mass.{{r|aebersold2003}} | 11 | It is a soft technique — the molecule survives largely intact — and it produces multiply charged ions from peptides and proteins, so a large molecule appears at a mass-to-charge ratio within the range of ordinary analysers. A single peptide therefore produces a series of peaks at successive charge states, deconvolved by software into one neutral mass.{{r|aebersold2003}} |
| 12 | 12 | ||
| + | 13 | Because it works from flowing solution, it couples directly to chromatography, which is what makes [[Liquid chromatography-mass spectrometry|LC-MS]] possible.{{r|fenn1989}} | |
| + | 14 | ||
| 13 | == Mechanism == | 15 | == Mechanism == |
| 14 | The applied potential draws the liquid into a cone from whose tip a fine spray of charged droplets emerges. Solvent evaporates, the droplets shrink until charge repulsion exceeds surface tension, and they divide. Ions are ultimately released either by field-driven ejection from very small droplets or by complete evaporation of the solvent around them.{{r|fenn1989}} | 16 | The applied potential draws the liquid into a cone from whose tip a fine spray of charged droplets emerges. Solvent evaporates, the droplets shrink until charge repulsion exceeds surface tension, and they divide. Ions are ultimately released either by field-driven ejection from very small droplets or by complete evaporation of the solvent around them.{{r|fenn1989}} |
| ⋮ | ⋮ | ||
| 24 | [[Category:Mass spectrometry]] | 26 | [[Category:Mass spectrometry]] |
| 25 | [[Category:Analytical methods]] | 27 | [[Category:Analytical methods]] |
| + | 28 | [[Category:Analytical science]] | |
| 26 | 29 |