Custom peptide synthesis (revision 4)
Old revision·22:34, 1 Nov 2024·CategoryBot
| Custom peptide synthesis | |
|---|---|
| Input | A sequence and a specification |
| Priced by | Length, scale, purity grade, modifications |
| Contrast with | Catalogue supply of stocked compounds |
| Topic infobox · conventions | |
Custom peptide synthesis is the manufacture of a peptide to a sequence and specification supplied by the customer, as distinct from supply of a catalogue item held in stock. It is the ordinary route by which research peptides that are not commercially stocked are obtained.[1]
A custom order is defined by more than a sequence. Scale, purity grade, C-terminal functionality, any modifications such as acylation or cyclisation, salt form, and which determinations will appear on the certificate are all specification items, and a quotation that does not enumerate them is quoting for an underdetermined thing.[2]
What a specification should fix
[edit]| Item | Why it matters |
|---|---|
| Sequence and modifications | Defines the molecule; ambiguity here is fatal |
| C-terminal form | Acid or amide; set by linker choice |
| Purity grade | Determines purification effort and price |
| Salt form | Trifluoroacetate counterion or exchanged; affects Peptide content |
| Scale | Net peptide mass, not solid mass |
| Determinations on the certificate | What will actually be reported |
The distinction between net peptide mass and solid mass is the one most often left ambiguous. An order for "10 mg" may be filled as 10 mg of solid, which at typical content is rather less peptide, or as solid containing 10 mg of peptide. The two differ by the counterion and water fractions.[2]
Sequence ambiguity is the failure with the largest consequence. One-letter and three-letter codes, D-residue conventions, and non-standard abbreviations for modified residues all vary between houses, and a sequence should be unambiguous on its face. See Peptide.