Crude peptide (revision 1)
Old revision·10:37, 12 Sep 2024·CDMO_Caradoc
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| Crude peptide | |
|---|---|
| Obtained from | Cleavage of the assembled chain |
| Typical purity | Highly variable; sequence-dependent |
| Principal impurities | Deletion and truncated sequences |
| Topic infobox · conventions | |
Crude peptide is the material obtained immediately after cleavage from the solid support, before any chromatographic purification. It contains the target peptide together with every by-product that accumulated during assembly and cleavage.[1]
Its purity is highly variable and is largely determined by sequence. An easy 10-residue sequence may cleave at high purity; a difficult 40-residue sequence may cleave at well under half. That figure sets the cost and difficulty of everything that follows.[1]
References
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